Reporter

Part:BBa_M36629:Design

Designed by: Benjamin Yeh, Jodie Sheffels, Ali Hoffer   Group: Stanford BIOE44 - S11   (2015-10-24)


FLAG Epitope


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Avoid BsaI, BbsI, and BsmBI restriction sites 2. Avoid repeats over 10bp in length.


Source

The DNA sequence was generated from the amino acid sequence (DYKDDDDK) using IDT Codon Optimizer for E. coli K12. It was then modified to remove certain restriction sites and repeats.

References