Reporter
Part:BBa_M36629:Design
Designed by: Benjamin Yeh, Jodie Sheffels, Ali Hoffer Group: Stanford BIOE44 - S11 (2015-10-24)
FLAG Epitope
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. Avoid BsaI, BbsI, and BsmBI restriction sites 2. Avoid repeats over 10bp in length.
Source
The DNA sequence was generated from the amino acid sequence (DYKDDDDK) using IDT Codon Optimizer for E. coli K12. It was then modified to remove certain restriction sites and repeats.